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Image Search Results
Journal: Journal of Cancer
Article Title: Linc00662 sponges miR-15b-5p to promote hypopharyngeal squamous cell carcinoma progression by facilitating cancer stem cell-like phenotypes
doi: 10.7150/jca.95852
Figure Lengend Snippet: Linc00662 directly binds to miR-15b-5p in FaDu and 293T cells (A) The candidate miRNAs which could potentially target Linc00662 were listed according to miRDB, ENCORI and LncRNASNP2. (B) Expression of miR-15b-5p in 497 patients with HNSC and 44 normal samples. (C) The expression of miR-15b-5p in para-carcinoma and HSCC cancer tissues. (D) The association between miR-15b-5p and Linc00662 in HNSC was analyzed using ENCORI Starbase. (E) The association between miR-15b-5p and Linc00662 in HSCC tissues. (F) The expression of miR-15b-5p mRNA were assessed by qRT-PCR in FaDu cells. (G) Luciferase reporter assay was performed to verify the interaction of miR-15b-5p and Linc00662 in 293T cells. **P < 0.01.
Article Snippet: Approximately 1 μg of RNA from tissue was reverse transcribed using the
Techniques: Expressing, Quantitative RT-PCR, Luciferase, Reporter Assay
Journal: Journal of Cancer
Article Title: Linc00662 sponges miR-15b-5p to promote hypopharyngeal squamous cell carcinoma progression by facilitating cancer stem cell-like phenotypes
doi: 10.7150/jca.95852
Figure Lengend Snippet: Linc00662 / miR-15b-5p modulates HSCC growth in vivo. (A) Representative images of tumor tissues from the FaDu cells transfected with Linc00662 or the combination of Linc00662 and miR-15b-5p mimic for 72 h were obtained on day 7. Vector (n=7): FaDu cell transfected with the vector; LINC00662 (n=6): FaDu cell transfected with LINC00662 overexpression plasmid; LINC00662 +NC mimic (n=6): FaDu cell transfected with LINC00662 overexpression plasmid with negative control mimic; LINC00662 +miRNA mimic (n=7): FaDu cell transfected with LINC00662 overexpression plasmid with miR-15b-5p mimic. (B) Tumor volume was calculated every 3 d after injection. (C) Weight of the mouse body was measured at the end of modeling. (D) The expression level of Linc000662 in tumor tissue as determined using qRT-PCR. (E) The expression level of miR-15b-5p in tumor tissue as determined using qRT-PCR. *P < 0.05, **P < 0.01.
Article Snippet: Approximately 1 μg of RNA from tissue was reverse transcribed using the
Techniques: In Vivo, Transfection, Plasmid Preparation, Over Expression, Negative Control, Injection, Expressing, Quantitative RT-PCR